Quantification of polysaccharides fixed to Gram stained slides using lactophenol cotton blue and digital image processing
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منابع مشابه
Quantification of polysaccharides fixed to Gram stained slides using lactophenol cotton blue and digital image processing
Dark blue rings and circles emerged when the non-specific polysaccharide stain lactophenol cotton blue was added to Gram stained slides. The dark blue staining is attributable to the presence of capsular polysaccharides and bacterial slime associated with clumps of Gram-negative bacteria. Since all bacterial cells are glycosylated and concentrate polysaccharides from the media, the majority of...
متن کاملQuantification of polysaccharides fixed to Gram stained slides using lactophenol cotton blue and digital image
Dark blue rings and circles emerged when the non-specific polysaccharide stain lactophenol cotton blue was added to Gram stained slides. The dark blue staining is attributable to the presence of capsular polysaccharides and bacterial slime associated with clumps of Gram-negative bacteria. Since all bacterial cells are glycosylated and concentrate polysaccharides from the media, the majority of...
متن کاملQuantification of polysaccharides fixed to Gram stained slides
I discovered indigo rings and circles in ATCC® 25922TM Escherichia coli cultures when I added the non-specific polysaccharide stain lactophenol cotton blue to Gram stained slides sampled from 96-well plates used to measure quantitative growth kinetics (QGK) in virtual colony count antimicrobial assays. I attribute the dark blue staining to the presence of capsular polysaccharides and bacterial ...
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ژورنال
عنوان ژورنال: F1000Research
سال: 2017
ISSN: 2046-1402
DOI: 10.12688/f1000research.5779.3